fast delivery Guanidine Thiocyanate593-84-0 on hot sellinghigh quality 593-84-0 CAS NO.593-84-0

Guanidine thiocyanate is used for RNA isolation and can solubilize proteins. When Guanidine Thiocyanate is used to solubilize proteins it can be removed by dialysis against urea without difficulty. The hybridization of guanidine thiocyanate-solubilized hepatocytes by Guanidine Thiocyanate can be used as a method to quantify mRNA from hepatocytes. Blood sample lysate stored in Guanidine Thiocyanate has been shown to maintain nucleic acid integrity. Research describes Guanidine Thiocyanate to be ineffective for RNA isolation of plant tissue with a high levels of secondary metabolites and storage proteins.

Guanidine thiocyanate is one of the cheapest and easiest to prepare of the guanidine salts. Guanidine Thiocyanate is an ultrapure, molecular biology grade reagent. It is free of detectable nuclease and protease activity and is a strong protein denaturant, as both the guanidinium cation and the thiocyanate anion are chaotropic agents. It is provided in one bottle containing 500 g. It is recommended for isolation of RNA, especially for tissues such as pancreas with high levels of RNase activity. In solution, it is known as guanidinium thiocyanate. Guanidine Thiocyanate is thoroughly tested for contaminating nonspecific endonuclease, exonuclease, and RNase activity.

Guanidine thiocyanate is used as a chaotropic reagent in cell lysis buffers as it disrupts cell and organelle membranes. Guanidine thiocyanate is especially suitable for purification of nucleic acids from crude cell lysates as it is a protein denaturant, allowing for separation of nucleic acids from the protein fraction. Guanidine thiocyanate also inactivates DNAses and RNAses, giving favourable yields of nucleic acids.